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首页> 外文OA文献 >Use of Amplified Ribosomal DNA Restriction Analysis for Identification of Ralstonia and Pandoraea Species: Interest in Determination of the Respiratory Bacterial Flora in Patients with Cystic Fibrosis
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Use of Amplified Ribosomal DNA Restriction Analysis for Identification of Ralstonia and Pandoraea Species: Interest in Determination of the Respiratory Bacterial Flora in Patients with Cystic Fibrosis

机译：扩增的核糖体DNA限制性内切酶分析用于鉴定罗尔斯通氏菌和潘多拉菌种：对囊性纤维化患者呼吸细菌菌群测定的兴趣

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摘要

The recovery of Ralstonia and Pandoraea species from respiratory tract cultures of patients with cystic fibrosis has recently been reported. These species are difficult to identify, and especially to differentiate from Burkholderia cepacia complex organisms, with classical methods. The discriminatory power of amplified ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by using a panel of six enzymes already proven suitable for the identification of Burkholderia species. ARDRA provided differentiation of all the Ralstonia species tested and of Pandoraea norimbergensis. Pandoraea species P. pnomenusa, P. sputorum, P. pulmonicola, and P. apista were not discriminated to the species level. This method allowed the identification of five clinical isolates recovered from French cystic fibrosis patients as Ralstonia mannitolilytica.
机译：最近已经报道了从囊性纤维化患者的呼吸道培养物中恢复了Ralstonia和Pandoraea种类。这些物种很难用经典方法鉴定，尤其是很难与洋葱伯克霍尔德菌复杂生物区分开来。通过使用一组已经证明适合鉴定伯克霍尔德菌属物种的六种酶，比较每个物种的参考菌株的限制性谱，评估了两个属中的扩增核糖体DNA限制性分析（ARDRA）的区分能力。 ARDRA提供了所测试的所有Ralstonia物种和pandoraea norimbergensis的区分。潘多拉亚物种P. pnomenusa，P。sputorum，P。pulmonicola和P. apista并未在物种水平上加以区分。这种方法可以鉴定出从法国囊性纤维化患者中回收的五个临床分离株，即Ralstonia mannitolilytica。

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